Dr Holder graduated from the University of the West Indies in 1985. Her Dermatology training took place at the St Johns Institute of Dermatology and the Royal London Hospital. She completed her specialist training in1997 and became a Fellow of the Royal College of Physicians in 2001. She obtained a diploma in the management of Allergic diseases from the University of Southampton in 2002.
Her first Consultant post was at the Royal Berkshire Hospital in 1998 and her second at the Calderdale and Huddersfield NHS Trust in West Yorkshire. She relocated south to take up a Consultant position and to work with former colleagues at the Basingstoke and North Hampshire Trust in January 2009.